Conference Schedule

Day1: March 26, 2018

Keynote Forum

Biography

Sebastian Ziewer Arndts completed his Diploma in Biology at University of Bonn from 2000-2006; he was scientific employee from 2006-2012 and completed his PhD thesis at University Hospital Bonn in 2012. His thesis was about “Immunological mechanisms and vaccination strategies in the murine model of human lymphatic filariasis”. He has been Trainer and Technical Consultant for XRF, NIR, FT-IR, and Raman spectroscopy at Bio-Chem division of Analyticon Instruments GmbH since 2012. He focuses on “Quality control in the chemical and pharmaceutical industry”.

 


Abstract

Molecular spectroscopy is used along the entire process chain in the chemical and pharmaceutical industry, e.g., for raw material identification in the warehouse, real-time monitoring in the production and batch release for final product control. This talk illustrates the most recent trends of NIR and Raman spectroscopy: Spatially offset Raman for raw material identification - portable spatially offset spectrometers are able to analyse substances without sampling in unopened containers, even if those are opaque like brown paper sacks or white plastic containers. Measurements can be done by non-expert users and are completed within seconds. Virtual split Raman for static inprocess control - the virtual split technology multiplies the photon yield tenfold. Measurements are shortened to seconds instead of minutes or hours and analytics in the ppm range are possible, perfect for quality-by-design and continuous manufacturing approaches. Linear variable filter NIR for dynamic in-process control - tiny NIR systems with linear variable filter technology have no moving parts or delicate fiber optics, and can be installed almost everywhere to monitor typical processes like granulation or drying. A wireless battery-powered version can be mounted in dynamic processes, like rotating blenders. Transmission Raman for final batch release-transmission Raman spectroscopy (TRS) has emerged as a non-invasive and consumables-free alternative to time-consuming and expensive HPLC. It’s mainly used for the analysis of tablets, e.g. the content of active pharmaceutical ingredient or polymorphs.

Biography

Emmanuel Mikros is Professor of Pharmaceutical Chemistry at University of Athens. He completed his PhD in Chemistry at Université Paris-Sud (Paris XI) in 1988 and then he joined University of Athens. He is a Research Fellow at Universität zu Lübeck, (Germany), and at INRA, Nantes, (France). He was awarded the DAAD (Germany, 1996) and Marie Curie (European 1994) Fellowships. He is co-author of 115 peer reviewed scientific publications. He has participated in over 20 funded research and projects. He is President of Hellenic Society of Medicinal Chemistry; invited speaker in more than 40 conferences and universities; he has also participated in the organizing committee of seven international and national scientific congresses. He leads a research group focused on NMR spectroscopy; NMR based metabolomics drug discovery and structure based drug design.

 

 


Abstract

Spectral data reflect concentration variations of the components of an extract and can correlate statistically with measurable dose-dependent properties such as bioactivity. A carefully planned fractionation of plant material can generate a concentration variance of the components and can be reflected on the corresponding spectroscopical data. Correlation of these data with bioactivity would result to the identification of active constituents in the complex extract or fraction mixtures prior to any purification step. Examples of the fractionation procedure using the Centrifugal Partition Chromatography technique (CPC) and the correlation of NMR and MS data with bioactivity to identify the active constituents through the heterocovariance statistical analysis will be discussed. This highly innovative activity-based-metabolite-profiling can dramatically accelerate the discovery of active natural products challenging global biodiversity and chemo-diversity.

Tracks

  • Novel Approaches to Analytical and Bioanalytical Methods | Analytical Methodology | NMR Analysis of Complex Natural Samples | Applications of Analytical and Bioanalytical Methods | Chemometrics | Environmental Analytical Chemistry
Location:

Sebastian Ziewer Arndts

Analyticon Instruments GmbH, Germany

Chair

Vusumzi E Pakade

Vaal University of Technology, South Africa

Co Chair

Biography

Svetlana Kuznetsova has completed his Candidate’s and Doctor’s dissertations at Siberian State University of Science and Technology. She has worked as Chief Researcher at Institute of Chemistry and Chemical Technology SB-RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS” and as Professor of Analytical and Organic Chemistry Department of Siberian Federal University. She has published more than 60 papers inreputed journals and 35 patents an invention.


Abstract

Extractive compounds of outer birch bark contain up to 40% of the mixture of pentacyclic triterpenoids and betulin is the main of them. Medical properties of birch bark extracts have been known for a long time in folk medicine. We suggested new methods of synthesis betulin, allobetulin and acyl derivatives of betulin directly from birch bark without preliminary separation of betulin by combined stages of betulin extraction and its acylation with acetic, propionic or butyric acids. The structure of betulin derivatives have been identified with chromatography and mass spectrometry, FTIR and NMR spectroscopy. It was found that this compound exhibits a broad range of biological activity, including its applications as an anti-cancer agent. The purpose of this work was to obtain fine products from birch bark and study their applications in medical and veterinary sciences.

Biography

Peter de Boves Harrington completed his PhD in Analytical Chemistry at University of North Carolina where he worked on “Artificial intelligence methods applied to analytical chemistry”. He then held a Research Professorship at Colorado School of Mines where he developed algorithms for identifying bacteria from their mass spectra. Afterwards, he started his career at OHIO where he now directs the Center for Intelligent Chemical Instrumentation. He has invented many chemometric algorithms that are in use today. Recently, he was honored with the Outstanding Research Award for his college and was named a recipient of China’s 1,000 Talents. He has published more than 170 papers in peer reviewed journals and is a world-renowned speaker.


Abstract

Many advances in modern society are due to embedded artificial intelligence. Some of these innovations are voice recognition in phones and personal assistants, image recognition for identifying faces in photos, and digital transcription of handwriting. These advances rely on deep neural networks, some of which are efficiently built with layers of restricted Boltzmann machines (RBMs). RBMs have many potential applications in analytical chemistry for finding features in data. They may be used to transform data and improve the performance for classification and calibration. Some examples will demonstrate the improvement form quantifying fat, moisture, and protein content using nearinfrared spectroscopy. Classification of spectra from botanical materials such as cannabis or tea is useful for authentication. The use of RBMs to improve the classification results also shall be demonstrated.

Biography

Beibei Zhang has completed his Master’s degree at Henan Agricultural University. He is a Doctor of the State Key Laboratory of Bioelectronics, Southeast University, a double first-rate university with world first-rate disciplines. He has published several papers in reputed journals with impact factor more than 15.


Abstract

This study develops a new method for detecting target DNA based on Cas9 nuclease, which was named as CARP, representing Cas9/sgRNAs-Associated Reverse PCR. This technique detects target DNA in three steps: cleaving the detected DNA sample with Cas9 in complex with a pair of sgRNAs specific to target DNA; ligating the cleaved DNA with DNA ligase and; amplifying target DNA with PCR. In the ligation step, the Cas9-cut target DNA was ligated into intramolecular circular or intermolecular concatenated linear DNA. In the PCR step, the ligated DNA was amplified with a pair of reverse primers. The technique was verified by detecting HPV16 and HPV18 L1 genes in nine different human papillomavirus (HPV) subtypes. The technique also detected the L1 and E6-E7 genes of two high-risk HPVs, HPV16 and HPV18, in the genomic DNA of two HPV-positive cervical carcinoma cells (HeLa and SiHa), in which no L1 and E6-E7 genes were detected in the HPV-negative cervical carcinoma cell, C-33a. By performing these proof-of-concept experiments, this study provides a new CRISPR-based DNA detection and typing method. Especially, the CARP method verified by this study is ready for the clinical HPV detection.

Biography

Shuyan Zhang has completed his Master’s degree from Zhejiang University. He is a Doctor of State Key Laboratory of Bioelectronics, Southeast University, China.


Abstract

It has been well-known that over activation of NF-κB has close relationship with hepatitis and hepatocellular carcinoma (HCC). However, the complete and exact underlying molecular pathways and mechanisms still remain not fully understood. By manipulating NF-κB activity with its recognized activator TNFα and using ChIP-seq and RNA-seq techniques, this study identified 699 NF-κB direct target genes (DTGs) in a widely used HCC cell line, HepG2, including 399 activated and 300 repressed genes. In these NF-κB DTGs, 216 genes (126 activated and 90 repressed genes) are among the current HCC gene signature. In comparison with NF-κB target genes identified in LPS-induced THP-1 and TNFα-induced HeLa cells, only limited numbers (24-46) of genes were shared by the two cell lines, indicating the HCC specificity of identified genes. Functional annotation revealed that NF-κB DTGs in HepG2 cell are mainly related with many typical NF-κB-related biological processes including immune system process, response to stress, response to stimulus, defense response, and cell death, and signaling pathways of MAPK, TNF, TGF-beta, Chemokine, NF-kappa B, and Toll-like receptor. Some NF-κB DTGs are also involved in Hepatitis C and B pathways. It was found that 82 NF-κB DTGs code secretory proteins, which include CCL2 and DKK1 that have already been used as HCC markers. Finally, the NF-κB DTGs were further confirmed by detecting the NF-κB binding and expression of 14 genes with ChIP-PCR and RT-PCR. This study thus provides a useful NF-κB DTG list for future studies of NF-κB-related molecular mechanisms and theranostic biomarkers of HCC.

Biography

Haojun Jin completed his Bachelor degree in Applied Chemistry in 2008 from Nanjing Agricultural University. He completed his Master degree in Organic Chemistry in 2011 from Nanjing Tech University. In 2013, he started his Doctoral program in State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, supervised by Professor Qingjiang Sun. As planned, he will complete Doctoral degree in 2018. His dissertation mainly focuses on design and construction of indicator displacement assay, based quantum dots-ligand probes, which are applicable for biomarkers. Until now, he has authored three papers in the esteemed international journals.


Abstract

Indicator displacement assay (IDA) have attracted tremendous interest recently because of their advantages of improved selectivity, high sensitivity and visibility for detection of diverse analytes such as anions, amino acids, saccharides and other functional group molecules. With a configuration of indicator-receptor, the analyte is detectable upon its competitive binding with receptor, thus giving rise to a new signal for the released indicator. Quantum dots (QDs) are a class of inorganic fluorophores with high fluorescence quantum yields, size-tunable narrow emission, and exceptional resistance to photo bleaching. Therefore, the QDs are one of the best candidates to serve as indicators in IDAs. In this work, we report quantum dots-ligand fluorescent probes for sensitive and selective detection of biomarkers, including G-quadruplex and avidin, based on IDA. In the IDA approaches, red-emission quantum dots (rQDs) deposited on silica coated green-emission quantum dots (gQDs) are indicators, while ligands (DI for G-quadruplex and PB for avidin) are receptors. The non-aqueous soluble receptors are facilely attached onto the surface of rQDs via phase transfer, and inducing the fluorescence quenching of rQDs in the mechanism of charge transfer (CT). As a key element in the IDAs, displacement of the receptors (ligands) can take place in the presence of trace concentrations of biomarkers due to their stronger affinity, breaking up the QDs-ligand complexation. Consequently, theCT processes are eliminated and the fluorescence of rQDs is restored. However, the fluorescence of gQDs stays constant due to the protection of silica in both of the IDAs. By measuring the fluorescent intensities of gQDs and rQDs, the ratiometric fluorescent response, which is extremely useful for improving the sensitivity and reliability, is achieved. Moreover, variations of dual-emission intensity ratios in the IDAs result in the continuous solution color changes, with benefits of which, naked-eye quantifications of the biomarkers are realized.

Biography

Xiaojun Qu completed his Bachelor degree and Master degree from College of Veterinary Medicine-Northwest A&F University, China in 2012. In 2014, he started his Doctoral program at State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering-Southeast University, supervised by Professor Qingjiang Sun. As planned, he will complete his Doctoral degree in 2019. His dissertation mainly focuses on the isothermal amplification reaction on different kinds of quantum dot-encoded bead, which are applicable for ultrasensitive detection of biological molecules. Until now, he has joined and finished two projects and has co-authored three papers on the esteemed international journals.


Abstract

MicroRNAs (miRNAs) are a group of small size (19–25 nucleotides in length), non-coding RNAs that play regulating roles in many critical biological processes. Recently, accumulating evidence indicates that the aberrant expression of miRNAs is associated with cancer initiation, tumor stage. Thus, miRNAs have been regarded as bio-markers in cancer diagnose. However, the unique characteristics of miRNA, such as their small size, sequence homology among family members, and low abundance, make miRNA more challenging to be analyzed. This article report a protocol of direct implementation of nicking enzyme assisted strand displacement amplification (SDA) on quantum dot-encoded core-shell silica beads (Qbead) and its application for multiplexed assay of human liver carcinoma miRNAs. Qbeads with remarkably improved chemical and photo-stability allows efficient bio-conjugation, hybridization and SDA on the surface. Multifunctional hairpin probes were used, which contain capture sequence specific target binding, nicking enzyme recognition site for the exponential amplification, the G-rich segment as the signal reporter with [Ru(bpy)2(DI)]2+ (Ru-DI), and 3’-end sequence triggering the strand-displacement amplification reaction. By the presence of target and enzyme, using molecular switch Ru-DI, the bead-SDA assay significantly improves the detection sensitivity. A dynamic range of 5 logs and a detection limit of 0.5 fM are achieved, owing to SDAinduced fluorescence signal amplification. More importantly, the SDA on Qbead results in color difference amplification allows color analysis be a conceivable quantification method for the Qbeads-based assay. With benefits of SDA-induced color difference amplification, quantify multiplex miRNAs in cancer cell lysates realized. The Qbead-SDA assay with color analysis could potentially enable the development of point-ofcare diagnostics platforms.

Biography

Hyeseon Lee completed her MS in Statistics department at Cornell University, and PhD at Kyungpook National University in Korea. She had career asa Research Programmer at National Opinion Research Center affiliated at University of Chicago, and as a Statistician in Medical School at University of California, San Diego. Her research interest is feature extraction method for high dimensional data, and applies to chemometrics. Her research work is prediction and classification adopting novel feature method, and also capturing canonical correlation structure. She is a Research Associate Professor in Department of Industrial and Management Engineering at Pohang University of Science and Technology (POSTECH).


Abstract

Feature extraction method for spectroscopy is essential for prediction and classification of mixture of compound material. Supervised preserve embedding method is applied for near-infra red or Raman spectra, and the accuracy of geographical origin of agricultural samples enhances. For precision, kernel-based calibration models are combined with multivariate feature selection. Kernel partial least square method and kernel-based support vector regression method are proposed for the specific chemical compositions.

Biography

Philiswa N Nomngongo completed her PhD in Chemistry (specializing in Analytical Chemistry) and Post-doctoral studies from University of Johannesburg, South Africa. She has worked as a Lecturer and Professor of Analytical Chemistry at University of Johannesburg. Her main research objective is to develop and apply different sample preparation methods for extraction and pre-concentration of trace organic and inorganic analytes in different sample matrices prior to the chromatographic or spectrometric determination. Her research also focuses on the application of nanotechnology for environmental pollution monitoring, desalination and water treatment. She has published more than 40 papers in highly rated and high impact international journals.


Abstract

A rapid and effective ultrasonic assisted-solid phase micro-extraction (UA-DSPME) was developed for the preconcentration of thallium in real water samples. The [email protected] AC composite functionalized with 1-(2-pyridylazo)- 2-naphthol (PAN) was used as an adsorbent and the target analyte was quantified using inductively coupled plasma optical emission spectrometry (ICP-OES). The morphological, crystalline physical and chemical properties of the composite were characterized by scanning electron microscope/energy dispersive X-ray spectroscopy (SEM/EDS), transmission electron microscope (TEM) and X-ray powder diffraction (XRD). The two-level fractional factorial design and response surface methodology were used to optimize experimental parameters affecting the pre-concentration procedure. Under optimum conditions, the enrichment factor (EF), limits of detection (LOD) and quantification (LOQ) were found to be 112, 0.25 ng L−1, and 0.84 ng L−1, respectively. Furthermore, intra-day and inter-day precisions expressed in terms of relative standard deviation (RSD) were found to be 2.4% and 4.3%, respectively. Moreover, the linear dynamic range was between LOQ and 350 μg L−1 with the correlation coefficient of 0.9966.

Biography

Vusumzi E Pakade is a Professor at Vaal University of Technology, South Africa. He has been working in the university from many years.


Abstract

Activated carbons derived from macadamia nutshell powderwere subjected to secondary modification by various concentrations of mineral acid. Subsequently, subjected to surface imprinting for the recognition of Cr(III). In a separate study, polymeric membrane sheets were modified by surface imprinting for Cr(III) removal. Non-imprited versions of both formats were prepared to use as controls. It has been noted that during removal of Cr(VI) by activated carbons (AC), part of Cr(VI) is reduced to Cr(IIII) and in turn not adsorbed on the surface. Therefore, the current study seeks to address the un-adsorbed Cr(III) by introducing the selective imprinting method for the recognition of Cr(III). FTIR, TGA, SEM revealed that the acid treatment modified the surface of the adsorbent. Compared to the raw AC the acid functionalized AC showed acidic characteristic bands. Fourier Transform Infrared Spectroscopy (FTIR) detected the presence of C=O, O-H and COO- functional groups on the surface of the modified AC. Brunauer-Emmett-Teller (BET) showed an increase in surface area from 549 to 582 m2 g-1 following treatment. Different experimental parameters were optimized for effective adsorption of Cr(VI). The optimal parameters were as follows: pH 1, solution concentration 100 mg L-1, reaction time 8 hours and adsorbent dosage 0.32 g indicating that adsorption is strongly dependent on the mentioned parameters.

Biography

Moustafa Khalifa has completed his PhD in Chemistry from Institute of Industrial Organic Chemistry, Poland Academy of Science, Warsaw, Poland, in 1982. He is a Lab Consultant for Drug and Food Quality Control Laboratories, Ministry of Health, Kuwait and has been serving as a Professor of Pesticides Chemistry and Analysis at Kafrelsheikh University, Egypt.


Abstract

It is well known that there are currently three synthetic phosphodiesterase type 5 (PDE-5) inhibitors, sildenafil citrate (brand name Viagra) (S), vardenafil hydrochloride (brand name Levitra) (V) and tadalafil (brand name Cialis) (T) that have been evaluated as an orally effective drugs for the treatment of ED since 1998. An adulteration of “natural” herbal products with at least one of the previously mentioned synthetic PDE- 5 inhibitors or their structurally analogue has been reported since 2001. It is true that adulteration of natural herbal products with undeclared synthetic PDE-5 inhibitors, or their structurally analogues is a growing trend and poses a health threat to consumers. The aim of this study was directed to investigate the presence of synthetic PDE-5 analogues illegally adulterated in natural herbal products intended to be used for improve male sexual potency in Kuwait. The samples evaluated in this study consisted of a pre-market herbal product (tablet or capsules forms) which has been a subject for registration by Kuwait Drug and Food Administration (KUDFA) as natural herbal products for improving sexual performance for man in the period from 2003 to 2012. In our laboratories identification of these analogues were done using, TLC, HPLC—PDA, HPLC— PDA¾ESI(+)¾MS and HPLC¾PDA¾ESI¾(+)¾MS/ MS HRMS, and NMR as analytical tools. Our results were indicated the presence of hydroxyhomosildenafil and thiosildenafil as analogous for sildenafil and aminotadalafil as analogue of tadalafil, that were adulterated in some investigated samples of herbal products. According to KUDFA rules all adulterated herbal products have been cancelled from registration and banned to be used by men in Kuwait.

Day2: March 27, 2018

Keynote Forum

Biography

Sebastian Ziewer Arndts completed his Diploma in Biology at University of Bonn from 2000-2006; he was scientific employee from 2006-2012 and completed his PhD thesis at University Hospital Bonn in 2012. His thesis was about “Immunological mechanisms and vaccination strategies in the murine model of human lymphatic filariasis”. He has been Trainer and Technical Consultant for XRF, NIR, FT-IR, and Raman spectroscopy at Bio-Chem division of Analyticon Instruments GmbH since 2012. He focuses on “Quality control in the chemical and pharmaceutical industry”.

 

 


Abstract

Efficiency is essential in the field of pharmaceutical production as regulatory guidelines are getting tougher and tougher. Therefore processes need to be optimized in order to use the given resources in the best possible way. The new and unique transmission Raman-spectrometer TRS100 ensures increased product safety during content uniformity tests, while simultaneously saving 60-90% of operating costs compared to the commonly used HPLC. With the TRS, the measurement time for one sample is under one second. Measurement itself is completely non-destructive, making considerably more measurements possible and substantially reducing release time. In contrast to HPLC the analysis is completely non-destructive, needs to sample preparation and the results are not user dependent, but even transferable from one instrument to another. This new and modern analytical approach to content uniformity testing will also be helpful when it comes to continuous manufacturing. Integrating such a device into a process will ensure that the quality is consistent and the final release can easily and reliably be done. In addition to the high through put and the time saving approach the instrument has only very little maintenance costs and no consumables in contrast to the normal HPLC analysis that is very costly. As Raman is also sensitive for polymorphous substances this kind of testing is also useful for this. The speech shows how this new approach can be a revolution for the pharmaceutical world as far as cost and time saving is concerned. In addition, it is explained how the transmission technology ensures extremely selective results and how easy model building is.

Biography

Vusumzi E Pakade has completed his PhD in Environmental Analytical Chemistry at University of the Witwatersrand. His PhD was on the synthesisn and application of molecularly imprinted polymers for trace metal removal and recovery of organic compounds from wastes. He has worked as a Senior Lecturer at Vaal University of Technology, South Africa. He has published more than 25 papers and book chapters in reputable journals and has been serving as a research representative in the Faculty Research and Innovation Committee.


Abstract

Hexavalent chromium (Cr(VI)) is toxic and carcinogenic to humans and plants. Its sources in nature include discharge from electroplating, chrome mining, paints and pigment, leather tanning, and textile industries. Methods such as chemical precipitation, reverse osmosis, ion exchange, adsorption and coagulations have been used for the removal of Cr(VI) from the environment. Among these, adsorption methods have enjoyed widespread acceptance due to their simplicity, cost effectiveness and offer fine-tuning of functional groups on the surface of adsorbents. To that extent, numerous adsorbents have been developed from synthetic polymers, biomass, clays, and natural polymers. Even though these adsorbents have shown promising applications but there has been challenges with regards to Cr(VI) removal. One of those challenges is the highly oxidative nature of Cr(VI) which tend to get reduced to its trivalent form (Cr(III)) during adsorption leading to incomplete removal of total chromium. Our studies have thus focused on developing adsorbents for the removal of both forms of chromium, sequentially or simultaneously. Biomass based adsorbents and ion imprinted polymers have been utilized in this study. Various modifications of Macadamia nutshells and mango kernels were explored for the removal of chromium and adsorption performance improved following ligand immobilization.

Tracks

  • Environmental Analytical Chemistry | Modern Pharmaceutical Analysis | Chiral Columns Chromatography | Chromatography as a Separation Technique | Applications of Analytical and Bioanalytical Methods | Analytical Methodology | Advances in Separation Techniques
Location:

Moustafa Khalifa

Drug and Food Quality Control Laboratories, Kuwait

Chair

Philiswa N Nomngongo

University of Johannesburg, South Africa

Co Chair

Biography

Vladimir Yu Guskov completed his Graduation with honors from magistracy of Chemical Faculty of Bashkir State University in 2009, and in 2012 he also defended his PhD thesis in the specialty Physical Chemistry. Now, he works at Faculty of Chemistry of the Bashkir State University as an Associate Professor. He heads the scientific direction of chromatography at Analytical Chemistry Chair. He has 21 scientific papers in Scopus-indexed journals. He works on the novel chiral columns for chromatography, based on supramolecular chirality phenomenon, stationary phase polarity estimation approaches and inverse gas chromatography application to study supramolecular structures properties.


Abstract

Novel chiral stationary phases design is one of the top challenges in modern chromatography. In last decade nanoscale structured materials have become promising because of their high enantioselectivity. But in any cases, chiral recognition is based on the molecular chirality of the stationary phase suggested. In the present work, it was proposed to use for the first time the stationary phases based on supramolecular structures of achiral compounds with induced chirality for the chromatographic separation of enantiomers. Two-dimensional supramolecular structures of uracil and its derivatives, melamine and cyanuric acid were used. As initial adsorbent to modify graphitized carbon black, inert solid support and porous polymers were used. A special method of inducing chirality based on Kondepudi effect was developed. Packed columns for GC and HPLC as well as capillary GC SCOT columns were designed. It was shown, that chiral columns can separate enantiomers of halo alkanes, alcohols, esters and terpenes. High enantioselectivity  was achieved when low-boiling racemates were separated. For 2-bromobutane the selectivity factor α>3 was observed. Itis the greatest value of α achieved in 2-bromobutane racemate separation. For 2-chlorobutane α=2.17 is also the greatest in all 2-chlorobutane separations. Such good separation was caused by the fact that one of enantiomers is adsorbed predominantly within the cavity of the supramolecular structure and another one is adsorbed on its surface. Separation on the proposed chiral stationary phase is characterized by the relative standard deviation of retention volumes not more than 7%. The stationary phases suggested showing good stability, as well as thermostability, and stability in normal phase HPLC. The financial support of the Russian Science Foundation (grant 17-73-10181) is gratefully acknowledged.

Biography

Tadeusz MichaÅ‚owski is a chemist and physicist, by education. He was a professor at Jagiellonian University and Cracow University of Technology, Poland; now retired. He (a) discovered  Generalized Electron Balance (GEB), considered as the law of Nature, formulated (b) GATES/GEB, as the best thermodynamic approach to electrolytic redoxsystems, (c) generalized equivalent mass (GEM), and (d) Equilibrium Law, as the counterproposal for mass action law. His activity concerned analytical chemistry, electrochemistry, electroanalysis and chemometrics. His scientific activity was reckoned among the main achievements in Analytical Chemistry in Poland.  Author of ca 200 papers, articles, chapters and several books.


Abstract

Calculation of solubility of solid phases as precipitates in aqueous media occupies a prominent place in scientific literature. However, the erroneous patterns of reasoning and simplifications made in textbooks, e.g. in J. Chem. Educ., as well as in didactic materials offered in the Internet, based on the schemas resulting from the ubiquitous stoichiometry of the reaction are unacceptable, and give incorrect results. This kind of the subject knowledge was clearly confirmed in the series of relevant challenges, organized in Anal. Bioanal. Chem. Note that the active participants of the challengeswere not students, but scientists, in general. The correct approach, based on the rules of conservation and detailed physicochemical/thermodynamic knowledge on the system considered is realizable according to generalized approach to electrolytic systems, presented. All the qualitative and quantitative knowledge is involved in the balances and independent expressions for the equilibrium constants.

Biography

Ghazale Daneshvar Tarigh completed her PhD in Analytical Chemistry from University of Tehran, Iran in 2015. She completed BSc in Pure Chemistry at University of Zanjan, in 2003 and MSc under the direction of Prof. Yadollah Yamini at TMU and Prof. Ali Jabbari at KNTU in 2009. Her field of interest is the development of new extraction technologies, with an emphasis on miniaturized sample preparation methods and separation techniques.


Abstract

Sample preparation is an essential step of the analytical process. Solid phase extraction (SPE) is one of the most routinely used procedures for the separation and pre-concentration of a variety of compounds and elements from complex samples due to their well-known advantages, which include the high enrichment factor, good recovery, use of small quantities of organic solvents and the possibility of automation (off- or on-line) of the whole process. Carbon nanotubes (CNTs) have been proposed as a solid phase extractant for various inorganic and organic compounds at trace levels. In general, CNTs are insoluble in most common solvents, due to the aggregation induced by Van der Waals attractions. To optimize the potential applications of CNTs, it is essential to modify them with functional groups and/or nanoparticles in order to integrate the CNTs into desired structures or attach suitable nanostructures to them. One such approach is the magnetization of CNT with iron oxide nanoparticles. This article mention the different applications of magnetic carbon nanotube (MWCNT) in separation science such as pre-concentration and extraction, photo degradation, enantioseparation and speciation for inorganic and organic compounds in different matrices. Magnetic separation and pre-concentration using MWCNT provided optimum and selective sample pretreatment procedures that minimized the required sample preparation time, reduced the number of matrix manipulations and the sample-preparation steps, and avoided the introduction of sources of uncertainty in the analytical process.