The advancement of the bio analytical methodology brought a dynamic discipline for which the future holds many energizing opportunities to further advancements. The principle effect of bio analysis in the pharmaceutical area is to acquire a quantitative measure of the drug and its metabolites. The reason for existing is to play out the pharmacokinetics, toxic kinetics, bioequivalence and presentation reaction like pharmacokinetic/pharmacodynamics examines. Different bio analytical systems are performed in bio analytical studies, for example, hyphenated methods, chromatographic methods, and ligand bio analytical strategies. This audit widely highlights the part of bio analytical methods and hyphenated instruments in evaluating the bio analysis of the medications.