The advancement of the bioanalytical methodology brought a dynamic discipline for which the future holds many energizing opportunities to further advancements. The principle effect of bionalysis in the pharmaceutical area is to acquire a quantitative measure of the drug and its metabolites. The reason for existing is to play out the pharmacokinetics, toxicokinetics, bioequivalence and presentation reaction like pharmacokinetic/pharmacodynamic examines. Different bioanalytical systems are performed in bioanalytical studies, for example, hyphenated methods, chromatographic methods, and ligand bioanalytical strategies. This audit widely highlights the part of bioanalytical methods and hyphenated instruments in evaluating the bioanalysis of the medications.